confocal laser scanning micrographs (clsm) lsm 510 meta  (Carl Zeiss)

Journal: Microbiology Spectrum

Article Title: Phytic Acid Demonstrates Rapid Antibiofilm Activity and Inhibits Biofilm Formation When Used as a Surface Conditioning Agent

doi: 10.1128/spectrum.00267-23

Figure Lengend Snippet: Confocal laser-scanning microscopy (CLSM) of live (green)/dead (red)-stained 72-h E. faecalis ATCC 29212 mono-species biofilms that were developed on HA coupons and treated with water (a) or 1.25% (b), 2.5% (c), or 5% (d) IP6 for 5 min. The square images show biofilm projection through the x - y plane, the bottom rectangle shows the x - z projection, and the right rectangle shows the y - z plane. Scale bar, 20 μm. (e) Biomass (green channel and red channel) of the mono-species biofilms treated with IP6 for 5 min. (f) Biomass (green channel) relative percent reduction (compared to untreated biofilms) of the mono-species biofilms. Data are expressed as means of four independent experiments. Error bars represent SD; *, P < 0.05; **, P < 0.01; ****, P < 0.0001.

Article Snippet: CLSM micrographs were generated using Imaris viewer software (9.9.1 version).

Techniques: Confocal Laser Scanning Microscopy, Staining

Journal: Microbiology Spectrum

Article Title: Phytic Acid Demonstrates Rapid Antibiofilm Activity and Inhibits Biofilm Formation When Used as a Surface Conditioning Agent

doi: 10.1128/spectrum.00267-23

Figure Lengend Snippet: Confocal laser-scanning microscopy (CLSM) of live (green)/dead (red)-stained 72-h E. faecalis ATCC 29212 and C. albicans ATCC 90028 dual-species biofilms that were developed on HA coupons and treated with water (a) or 1.25% (b), 2.5% (c), or 5% (d) IP6 for 5 min. The square images show biofilm projection through the x - y plane, the bottom rectangle shows the x - z projection, and the right rectangle shows the y - z plane. Scale bar, 20 μm. The graphs below the CLSM images represent the distribution of pixel average of both stains along the biofilm thickness. Pixel average was calculated from three independent experiments. (e) Biomass (green channel and red channel) of the dual-species biofilms treated with IP6 for 5 min. (f) Biomass (green channel) relative percent reduction (compared to untreated biofilms) of the dual-species biofilms. Data are expressed as means of three independent experiments for CLSM and four independent experiments for CFU analysis. Error bars represent SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: CLSM micrographs were generated using Imaris viewer software (9.9.1 version).

Techniques: Confocal Laser Scanning Microscopy, Staining

Journal: Microbiology Spectrum

Article Title: Phytic Acid Demonstrates Rapid Antibiofilm Activity and Inhibits Biofilm Formation When Used as a Surface Conditioning Agent

doi: 10.1128/spectrum.00267-23

Figure Lengend Snippet: Dual-species biofilms of E. faecalis ATCC 29212 and C. albicans ATCC 90028 developed on HA coupons for 24 h that were either preconditioned with water (control) or 2.5% IP6. (a and b) Scanning electron microscopy (bottom) and confocal laser-scanning microscopy (top) images of live/dead-stained dual-species biofilms that were developed on HA coupons preconditioned with either water (a) or 2.5% IP6 (b) are shown. Red arrows indicate E. faecalis communities, while yellow arrows indicate C. albicans . (c) Graph bars represent the biomass of developed biofilms on HA coupons that were either preconditioned with water (control) or 2.5% IP6. Data are expressed as the means of three independent experiments. Error bars and values in parentheses represent SD; ****, P < 0.0001; ***, P < 0.001; ns, not significant.

Article Snippet: CLSM micrographs were generated using Imaris viewer software (9.9.1 version).

Techniques: Control, Electron Microscopy, Confocal Laser Scanning Microscopy, Staining

Journal: Saudi Journal of Biological Sciences

Article Title: Food color ‘Azorubine’ interferes with quorum sensing regulated functions and obliterates biofilm formed by food associated bacteria: An in vitro and in silico approach

doi: 10.1016/j.sjbs.2020.01.001

Figure Lengend Snippet: A. Inhibitory action of azorubine on the biofilm formation in tests bacteria using crystal violet assay. Data are represented as mean value of triplicate readings and bars show the SD. * p ≤ 0.05, ** p ≤ 0.005, *** p ≤ 0.001 as compared to untreated control. B. Light microscopy, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) images of untreated control and azorubine treated biofilm inhibition.

Article Snippet: CLSM micrographs were recorded using ZEISS LSM780, Germany.

Techniques: Bacteria, Crystal Violet Assay, Control, Light Microscopy, Electron Microscopy, Confocal Laser Scanning Microscopy, Inhibition